My first introduction to the beast was in my first job out of undergrad at the USDA ARS unit in Raleigh. My colleague and I took weekly measurements on soybean plants, and I was given rudimentary training in operating the instrument. I could turn it on, hit the measuring button when the numbers seemed somewhat stable, and collect the data off of it at the end of the day. I had no idea what it was doing when I hit a button, what to look for to make sure it was measuring properly, or why "matching" was important. We had few issues, though, so my limited knowledge was adequate for the job.
Two years later when I learned my PhD project would be using the 6400, I arrogantly expressed my competence with the using the instrument. Of course I knew how to work it. I had done so for six months at my old job. It's a breeze. HA! I was a child. I knew nothing about the beast. I could put it together, yes. And I could turn it on, yes. But then I was lost at the very first screen. The programs had different names and I didn't know what made them different or how to choose the right one.
During my first field season, I learned through trial and error. Repeatedly. Painfully. Exhaustingly. I had to remeasure the same 100 plants eight times because my limited knowledge resulted in bad data 😳. Eight. Times. A six-day experiment turned into a thirty-day experiment. But I was learning. That fall, I attended the LI-COR photosynthesis training workshops and my eyes were opened. I finally knew what "matching" was and why it was important. I learned what the different programs were on the opening screen and how to adjust those to my project. I could add prompts and remove remarks and set stability settings. (We also learned about the domesticated breed of this instrument, the LI-6800. If the 6400 is a wild beast, the 6800 is a lamb. Gentle and simple and easy to use in every way imaginable. But I digress.) After this training, I at last had the tools and knowledge to master the LI-6400XT.
One and a half field seasons later and I can confidently remove and clean the fluorometer head mirrors to stabilize erratic CO2 values. I can rig a marine battery to power the instrument for 12+ continuous hours in the field. And I can take apart the console, remove broken battery ports, solder new ones in place, and put it all back to together in a way that still works correctly afterwards (miraculously). I know when and how to calibrate various sensors, what buttons to push to check for funky numbers, and how to troubleshoot when something looks wrong. Additionally, LI-COR support is on speed dial for all the questions that will inevitably arise I still don't know how to fix.
I'm learning how to be a LI-COR tamer. It's not an easy task, but good data is well-worth the investment. And as with any beast, "if you treat it right, it will treat you right" (Kusum Naithani, 2018).
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